bio rad mca497g rabbit anti gfap Search Results


rat anti mouse f4 80  (Bio-Rad)
96
Bio-Rad rat anti mouse f4 80
Rat Anti Mouse F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti mouse f4 80/product/Bio-Rad
Average 96 stars, based on 1 article reviews
rat anti mouse f4 80 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
f4/80  (Bio-Rad)
90
Bio-Rad f4/80
F4/80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/f4/80/product/Bio-Rad
Average 90 stars, based on 1 article reviews
f4/80 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
alexa fluor 647-conjugated anti-mouse b220  (Bio-Rad)
90
Bio-Rad alexa fluor 647-conjugated anti-mouse b220
Effect of BBS1 gene deletion on L. major host infectivity.A. BALB/c mice were infected with L. major wild-type (WT), BBS1 null (BBS1−/−) and complemented (BBS1−/−[+BBS1]) lines by subcutaneous injection of 5 × 10 6 metacyclic promastigotes into the right hind footpad. Developing lesions were monitored over 60 days. Mean lesion thickness is shown ( n = 5) ± SD. Data presented here represent one of two independent experiments.B. Parasite burden was measured in three footpads from each group of infected mice as in (A), by a limiting dilution assay following termination. Mean parasite burden per footpad is shown, combining data from two independent experiments ( n = 6) ± SD. By this method, no parasites were detected in five of six mice infected with the L. major BBS1 null line.C. Immunofluorescence of lymph nodes draining the site of infection in BALB/c mice, 60 days post-infection with L. major parasite lines as above (two areas of the lymph node are shown for BBS1 complemented line). Tissue sections were probed with antibodies against L. major HASPB (yellow), the macrophage marker F4/80 (green) and B-cell marker <t>B220</t> (pink). Bar, 200 μm.D. Lymph node section from a mouse infected with BBS1 complemented line for 60 days, probed with anti-HASPB (yellow) and F4/80 (green) and co-stained with DAPI (blue). Bar, 20 μm.E. Infected mouse lymph node sections probed with anti-HASPB (yellow) and co-stained with DAPI (blue). Bar, 2.5 μm.F. Infected mouse lymph node sections probed with anti-HASPB (red) and anti-LAMP1 (green) and co-stained with DAPI (blue). Bar, 5 μm.G. Infected and naïve mouse lymph node sections probed with anti-HASPB (yellow), anti-acetylated α-tubulin (pink) and anti-tyrosinated α-tubulin (green) and co-stained with DAPI (blue). Bar, 20 μm.
Alexa Fluor 647 Conjugated Anti Mouse B220, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 647-conjugated anti-mouse b220/product/Bio-Rad
Average 90 stars, based on 1 article reviews
alexa fluor 647-conjugated anti-mouse b220 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rat igg1 anti-f4/80  (Bio-Rad)
90
Bio-Rad rat igg1 anti-f4/80
Effect of BBS1 gene deletion on L. major host infectivity.A. BALB/c mice were infected with L. major wild-type (WT), BBS1 null (BBS1−/−) and complemented (BBS1−/−[+BBS1]) lines by subcutaneous injection of 5 × 10 6 metacyclic promastigotes into the right hind footpad. Developing lesions were monitored over 60 days. Mean lesion thickness is shown ( n = 5) ± SD. Data presented here represent one of two independent experiments.B. Parasite burden was measured in three footpads from each group of infected mice as in (A), by a limiting dilution assay following termination. Mean parasite burden per footpad is shown, combining data from two independent experiments ( n = 6) ± SD. By this method, no parasites were detected in five of six mice infected with the L. major BBS1 null line.C. Immunofluorescence of lymph nodes draining the site of infection in BALB/c mice, 60 days post-infection with L. major parasite lines as above (two areas of the lymph node are shown for BBS1 complemented line). Tissue sections were probed with antibodies against L. major HASPB (yellow), the macrophage marker F4/80 (green) and B-cell marker <t>B220</t> (pink). Bar, 200 μm.D. Lymph node section from a mouse infected with BBS1 complemented line for 60 days, probed with anti-HASPB (yellow) and F4/80 (green) and co-stained with DAPI (blue). Bar, 20 μm.E. Infected mouse lymph node sections probed with anti-HASPB (yellow) and co-stained with DAPI (blue). Bar, 2.5 μm.F. Infected mouse lymph node sections probed with anti-HASPB (red) and anti-LAMP1 (green) and co-stained with DAPI (blue). Bar, 5 μm.G. Infected and naïve mouse lymph node sections probed with anti-HASPB (yellow), anti-acetylated α-tubulin (pink) and anti-tyrosinated α-tubulin (green) and co-stained with DAPI (blue). Bar, 20 μm.
Rat Igg1 Anti F4/80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat igg1 anti-f4/80/product/Bio-Rad
Average 90 stars, based on 1 article reviews
rat igg1 anti-f4/80 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
anti-f4/80-alexa647  (Bio-Rad)
90
Bio-Rad anti-f4/80-alexa647
Effect of BBS1 gene deletion on L. major host infectivity.A. BALB/c mice were infected with L. major wild-type (WT), BBS1 null (BBS1−/−) and complemented (BBS1−/−[+BBS1]) lines by subcutaneous injection of 5 × 10 6 metacyclic promastigotes into the right hind footpad. Developing lesions were monitored over 60 days. Mean lesion thickness is shown ( n = 5) ± SD. Data presented here represent one of two independent experiments.B. Parasite burden was measured in three footpads from each group of infected mice as in (A), by a limiting dilution assay following termination. Mean parasite burden per footpad is shown, combining data from two independent experiments ( n = 6) ± SD. By this method, no parasites were detected in five of six mice infected with the L. major BBS1 null line.C. Immunofluorescence of lymph nodes draining the site of infection in BALB/c mice, 60 days post-infection with L. major parasite lines as above (two areas of the lymph node are shown for BBS1 complemented line). Tissue sections were probed with antibodies against L. major HASPB (yellow), the macrophage marker F4/80 (green) and B-cell marker <t>B220</t> (pink). Bar, 200 μm.D. Lymph node section from a mouse infected with BBS1 complemented line for 60 days, probed with anti-HASPB (yellow) and F4/80 (green) and co-stained with DAPI (blue). Bar, 20 μm.E. Infected mouse lymph node sections probed with anti-HASPB (yellow) and co-stained with DAPI (blue). Bar, 2.5 μm.F. Infected mouse lymph node sections probed with anti-HASPB (red) and anti-LAMP1 (green) and co-stained with DAPI (blue). Bar, 5 μm.G. Infected and naïve mouse lymph node sections probed with anti-HASPB (yellow), anti-acetylated α-tubulin (pink) and anti-tyrosinated α-tubulin (green) and co-stained with DAPI (blue). Bar, 20 μm.
Anti F4/80 Alexa647, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-f4/80-alexa647/product/Bio-Rad
Average 90 stars, based on 1 article reviews
anti-f4/80-alexa647 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rat anti-brdu  (Bio-Rad)
90
Bio-Rad rat anti-brdu
No significant changes in numbers of proliferating <t>microglia</t> <t>(PCNA</t> + and <t>BrdU</t> + ) in the hilus of the hippocampal DG. (A) Proliferating cell nuclear antigen (PCNA; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (B) Bromodeoxyuridine (BrdU; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (C) No significant effects on the number of proliferating PCNA + microglia was observed. (D) The number of Iba-1 + BrdU + cells was also not affected during de- or remyelination in either age group. Values are shown as means + SD ( n = 6 per group). Statistical significance was evaluated using a two-way ANOVA and a Tukey’s post hoc test. Bars: (A) 100 μm.
Rat Anti Brdu, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-brdu/product/Bio-Rad
Average 90 stars, based on 1 article reviews
rat anti-brdu - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
monoclonal antibodies fa11 (anticd68  (Bio-Rad)
90
Bio-Rad monoclonal antibodies fa11 (anticd68
No significant changes in numbers of proliferating <t>microglia</t> <t>(PCNA</t> + and <t>BrdU</t> + ) in the hilus of the hippocampal DG. (A) Proliferating cell nuclear antigen (PCNA; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (B) Bromodeoxyuridine (BrdU; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (C) No significant effects on the number of proliferating PCNA + microglia was observed. (D) The number of Iba-1 + BrdU + cells was also not affected during de- or remyelination in either age group. Values are shown as means + SD ( n = 6 per group). Statistical significance was evaluated using a two-way ANOVA and a Tukey’s post hoc test. Bars: (A) 100 μm.
Monoclonal Antibodies Fa11 (Anticd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal antibodies fa11 (anticd68/product/Bio-Rad
Average 90 stars, based on 1 article reviews
monoclonal antibodies fa11 (anticd68 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rat anti-cd68  (Bio-Rad)
90
Bio-Rad rat anti-cd68
No significant changes in numbers of proliferating <t>microglia</t> <t>(PCNA</t> + and <t>BrdU</t> + ) in the hilus of the hippocampal DG. (A) Proliferating cell nuclear antigen (PCNA; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (B) Bromodeoxyuridine (BrdU; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (C) No significant effects on the number of proliferating PCNA + microglia was observed. (D) The number of Iba-1 + BrdU + cells was also not affected during de- or remyelination in either age group. Values are shown as means + SD ( n = 6 per group). Statistical significance was evaluated using a two-way ANOVA and a Tukey’s post hoc test. Bars: (A) 100 μm.
Rat Anti Cd68, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-cd68/product/Bio-Rad
Average 90 stars, based on 1 article reviews
rat anti-cd68 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rat monoclonal anti-neutrophil antibody  (Bio-Rad)
90
Bio-Rad rat monoclonal anti-neutrophil antibody
No significant changes in numbers of proliferating <t>microglia</t> <t>(PCNA</t> + and <t>BrdU</t> + ) in the hilus of the hippocampal DG. (A) Proliferating cell nuclear antigen (PCNA; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (B) Bromodeoxyuridine (BrdU; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (C) No significant effects on the number of proliferating PCNA + microglia was observed. (D) The number of Iba-1 + BrdU + cells was also not affected during de- or remyelination in either age group. Values are shown as means + SD ( n = 6 per group). Statistical significance was evaluated using a two-way ANOVA and a Tukey’s post hoc test. Bars: (A) 100 μm.
Rat Monoclonal Anti Neutrophil Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat monoclonal anti-neutrophil antibody/product/Bio-Rad
Average 90 stars, based on 1 article reviews
rat monoclonal anti-neutrophil antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit-anti-rat fibronectin (polyclonal)  (Millipore)
90
Millipore rabbit-anti-rat fibronectin (polyclonal)
Primary antibodies for immunohistochemistry or immunofluorescence staining
Rabbit Anti Rat Fibronectin (Polyclonal), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit-anti-rat fibronectin (polyclonal)/product/Millipore
Average 90 stars, based on 1 article reviews
rabbit-anti-rat fibronectin (polyclonal) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rat antibodies neutrophil marker gr1  (Bio-Rad)
90
Bio-Rad rat antibodies neutrophil marker gr1
Primary antibodies for immunohistochemistry or immunofluorescence staining
Rat Antibodies Neutrophil Marker Gr1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat antibodies neutrophil marker gr1/product/Bio-Rad
Average 90 stars, based on 1 article reviews
rat antibodies neutrophil marker gr1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
f4 80  (Bio-Rad)
93
Bio-Rad f4 80
Primary antibodies for immunohistochemistry or immunofluorescence staining
F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/f4 80/product/Bio-Rad
Average 93 stars, based on 1 article reviews
f4 80 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


Effect of BBS1 gene deletion on L. major host infectivity.A. BALB/c mice were infected with L. major wild-type (WT), BBS1 null (BBS1−/−) and complemented (BBS1−/−[+BBS1]) lines by subcutaneous injection of 5 × 10 6 metacyclic promastigotes into the right hind footpad. Developing lesions were monitored over 60 days. Mean lesion thickness is shown ( n = 5) ± SD. Data presented here represent one of two independent experiments.B. Parasite burden was measured in three footpads from each group of infected mice as in (A), by a limiting dilution assay following termination. Mean parasite burden per footpad is shown, combining data from two independent experiments ( n = 6) ± SD. By this method, no parasites were detected in five of six mice infected with the L. major BBS1 null line.C. Immunofluorescence of lymph nodes draining the site of infection in BALB/c mice, 60 days post-infection with L. major parasite lines as above (two areas of the lymph node are shown for BBS1 complemented line). Tissue sections were probed with antibodies against L. major HASPB (yellow), the macrophage marker F4/80 (green) and B-cell marker B220 (pink). Bar, 200 μm.D. Lymph node section from a mouse infected with BBS1 complemented line for 60 days, probed with anti-HASPB (yellow) and F4/80 (green) and co-stained with DAPI (blue). Bar, 20 μm.E. Infected mouse lymph node sections probed with anti-HASPB (yellow) and co-stained with DAPI (blue). Bar, 2.5 μm.F. Infected mouse lymph node sections probed with anti-HASPB (red) and anti-LAMP1 (green) and co-stained with DAPI (blue). Bar, 5 μm.G. Infected and naïve mouse lymph node sections probed with anti-HASPB (yellow), anti-acetylated α-tubulin (pink) and anti-tyrosinated α-tubulin (green) and co-stained with DAPI (blue). Bar, 20 μm.

Journal: Molecular Microbiology

Article Title: The Leishmania major BBSome subunit BBS1 is essential for parasite virulence in the mammalian host

doi: 10.1111/mmi.12383

Figure Lengend Snippet: Effect of BBS1 gene deletion on L. major host infectivity.A. BALB/c mice were infected with L. major wild-type (WT), BBS1 null (BBS1−/−) and complemented (BBS1−/−[+BBS1]) lines by subcutaneous injection of 5 × 10 6 metacyclic promastigotes into the right hind footpad. Developing lesions were monitored over 60 days. Mean lesion thickness is shown ( n = 5) ± SD. Data presented here represent one of two independent experiments.B. Parasite burden was measured in three footpads from each group of infected mice as in (A), by a limiting dilution assay following termination. Mean parasite burden per footpad is shown, combining data from two independent experiments ( n = 6) ± SD. By this method, no parasites were detected in five of six mice infected with the L. major BBS1 null line.C. Immunofluorescence of lymph nodes draining the site of infection in BALB/c mice, 60 days post-infection with L. major parasite lines as above (two areas of the lymph node are shown for BBS1 complemented line). Tissue sections were probed with antibodies against L. major HASPB (yellow), the macrophage marker F4/80 (green) and B-cell marker B220 (pink). Bar, 200 μm.D. Lymph node section from a mouse infected with BBS1 complemented line for 60 days, probed with anti-HASPB (yellow) and F4/80 (green) and co-stained with DAPI (blue). Bar, 20 μm.E. Infected mouse lymph node sections probed with anti-HASPB (yellow) and co-stained with DAPI (blue). Bar, 2.5 μm.F. Infected mouse lymph node sections probed with anti-HASPB (red) and anti-LAMP1 (green) and co-stained with DAPI (blue). Bar, 5 μm.G. Infected and naïve mouse lymph node sections probed with anti-HASPB (yellow), anti-acetylated α-tubulin (pink) and anti-tyrosinated α-tubulin (green) and co-stained with DAPI (blue). Bar, 20 μm.

Article Snippet: For indirect immunofluorescence, draining lymph node sections (10 μm thick) were fixed with 4% paraformaldehyde (w/v) and stained as described (Yurdakul et al ., ) using Alexa Fluor 488-conjugated anti-mouse F4/80 (1:200, AbDSerotec), Alexa Fluor 647-conjugated anti-mouse B220 (1:200, AbDSerotec), mouse monoclonal 6-11B-1 against acetylated α-tubulin (1:300, Sigma), rat monoclonal YL1/2 against tyrosinated α-tubulin (1:200, Abcam), mouse monoclonal H4A3 against LAMP1 (1:500, Abcam) and rabbit anti-HASPB 336 (Flinn et al ., ) (1:300).

Techniques: Infection, Injection, Limiting Dilution Assay, Immunofluorescence, Marker, Staining

No significant changes in numbers of proliferating microglia (PCNA + and BrdU + ) in the hilus of the hippocampal DG. (A) Proliferating cell nuclear antigen (PCNA; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (B) Bromodeoxyuridine (BrdU; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (C) No significant effects on the number of proliferating PCNA + microglia was observed. (D) The number of Iba-1 + BrdU + cells was also not affected during de- or remyelination in either age group. Values are shown as means + SD ( n = 6 per group). Statistical significance was evaluated using a two-way ANOVA and a Tukey’s post hoc test. Bars: (A) 100 μm.

Journal: Frontiers in Aging Neuroscience

Article Title: Age Influences Microglial Activation After Cuprizone-Induced Demyelination

doi: 10.3389/fnagi.2018.00278

Figure Lengend Snippet: No significant changes in numbers of proliferating microglia (PCNA + and BrdU + ) in the hilus of the hippocampal DG. (A) Proliferating cell nuclear antigen (PCNA; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (B) Bromodeoxyuridine (BrdU; green) and Iba-1 (red) labeling. Cell nuclei are stained with DAPI (blue). (C) No significant effects on the number of proliferating PCNA + microglia was observed. (D) The number of Iba-1 + BrdU + cells was also not affected during de- or remyelination in either age group. Values are shown as means + SD ( n = 6 per group). Statistical significance was evaluated using a two-way ANOVA and a Tukey’s post hoc test. Bars: (A) 100 μm.

Article Snippet: The following antibodies and dilutions were used: primary antibodies: rat anti-myelin basic protein (anti-MBP; 1:150, MCA409S, AbD Serotec), goat anti-Iba-1 (1:500, Ab107159, Abcam), rabbit anti-P2RY12 (1:500, 55043A, AnaSpec), rabbit anti-CD68 (1:500, AB125212, Abcam), rat anti-MHCII (1:100, 14-5321-82, eBioscience), rat anti-Marco (1:200, MCA1849, AbD Serotec), rabbit anti-TSPO (PBR; 1:250, Novus Biologicals, NBP1-95674), mouse anti-CD206 (1:200, Ab8918, Abcam), rabbit anti-CD163 (1:200, Bs-2527R, Bioss), mouse anti-PCNA (1:500, A1111, Santa Cruz), rat anti-BrdU (1:500, OBT003G, Serotec), and rat anti-F4/80 (1:150, MCA497G, BioRad).

Techniques: Labeling, Staining

Age-related differences in the number of proliferating microglia (PCNA + and BrdU + ) in the splenium of the CC. (A) PCNA (green) and Iba-1 (red) labeling. (B) BrdU (green) and Iba-1 (red) staining. (C) The number of proliferating PCNA + microglia was significantly increased in the middle-aged control group compared to the younger one. In young mice, there was a significant increase in PCNA + microglia after demyelination and 2 weeks of remyelination. Thus, age-related differences in PCNA + Iba-1 + cell numbers were found in all treatment groups. (D) The number of BrdU + microglia was higher in the middle-aged group after 1 week of remyelination (a similar tendency was also observed in the other two groups). De- or remyelination did not affect the number of BrdU + Iba-1 + microglia. Values are shown as means + SD ( n = 6 per group). Statistical significance was evaluated using a two-way ANOVA and a Tukey’s post hoc test. The p -values are indicated in the graphs: treatment effects within the same age group: **** p < 0.0001, age-related differences: # p < 0.05, ## p < 0.01 and ### p < 0.001. Bars: (A) 100 μm.

Journal: Frontiers in Aging Neuroscience

Article Title: Age Influences Microglial Activation After Cuprizone-Induced Demyelination

doi: 10.3389/fnagi.2018.00278

Figure Lengend Snippet: Age-related differences in the number of proliferating microglia (PCNA + and BrdU + ) in the splenium of the CC. (A) PCNA (green) and Iba-1 (red) labeling. (B) BrdU (green) and Iba-1 (red) staining. (C) The number of proliferating PCNA + microglia was significantly increased in the middle-aged control group compared to the younger one. In young mice, there was a significant increase in PCNA + microglia after demyelination and 2 weeks of remyelination. Thus, age-related differences in PCNA + Iba-1 + cell numbers were found in all treatment groups. (D) The number of BrdU + microglia was higher in the middle-aged group after 1 week of remyelination (a similar tendency was also observed in the other two groups). De- or remyelination did not affect the number of BrdU + Iba-1 + microglia. Values are shown as means + SD ( n = 6 per group). Statistical significance was evaluated using a two-way ANOVA and a Tukey’s post hoc test. The p -values are indicated in the graphs: treatment effects within the same age group: **** p < 0.0001, age-related differences: # p < 0.05, ## p < 0.01 and ### p < 0.001. Bars: (A) 100 μm.

Article Snippet: The following antibodies and dilutions were used: primary antibodies: rat anti-myelin basic protein (anti-MBP; 1:150, MCA409S, AbD Serotec), goat anti-Iba-1 (1:500, Ab107159, Abcam), rabbit anti-P2RY12 (1:500, 55043A, AnaSpec), rabbit anti-CD68 (1:500, AB125212, Abcam), rat anti-MHCII (1:100, 14-5321-82, eBioscience), rat anti-Marco (1:200, MCA1849, AbD Serotec), rabbit anti-TSPO (PBR; 1:250, Novus Biologicals, NBP1-95674), mouse anti-CD206 (1:200, Ab8918, Abcam), rabbit anti-CD163 (1:200, Bs-2527R, Bioss), mouse anti-PCNA (1:500, A1111, Santa Cruz), rat anti-BrdU (1:500, OBT003G, Serotec), and rat anti-F4/80 (1:150, MCA497G, BioRad).

Techniques: Labeling, Staining

Primary antibodies for immunohistochemistry or immunofluorescence staining

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy

doi: 10.1681/ASN.2019080827

Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining

Article Snippet: Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310) R&D Systems, MAB1757 (US) Lipocalin-2 (NGAL) (for double staining) Rabbit-anti-NGAL (polyclonal) Thermo Fisher Scientific, PA5–79590 (US) Cytokeratin (Pan CK) Mouse-anti-pan Cytokeratin (clone Lu5) Abcam, ab17155 (UK) Proliferation (Ki-67, MIB1) Mouse-anti-human (clone KI-67P) Dianova, DIA-505 (GER) Proliferation (PCNA) Mouse-anti-PCNA (clone PC10) Calbiochem/Merck, NA03 (GER) Sm22 α /Transgelin Rabbit-anti-Transgelin (polyclonal) Abcam, ab14106 (UK) Tamm–Horsfall protein Rabbit-anti-human THP (polyclonal) SantaCruz, sc-20631 (GER) TNFR1 Rabbit-anti-TNFR1 (polyclonal) Abcam, ab19139 (UK) TNFR2 Rabbit-anti-TNFR2 (clone EPR1653) Abcam, ab15116 (UK) Vimentin Mouse-anti-vimentin (clone V9) Dako/Agilent, M0725 (US) Open in a separate window α -SMA, α –skeletal muscle actin; AQ-2, aquaporin-2; CD, cluster of differentiation; GER, Germany; CHE, Switzerland; F4/80, EGF-like module–containing mucin-like hormone receptor–like 1; TIM-1, T-cell immunoglobulin and mucin domain 1; HAVCR, Hepatitis A virus cellular receptor; NGAL, neutrophil gelatinase–associated lipocalin, lipocalin-2; CK, cytokeratin; PCNA, proliferating cell nuclear antigen; SM22 α , Smooth muscle protein 22- α /Transgelin; THP, Tamm–Horsfall protein, uromodulin; TNFR, tumor necrosis factor receptor.

Techniques: Immunohistochemistry, Immunofluorescence, Double Staining